| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mycolicibacterium smegmatis | GTTCACCGTTGCGAACCTG | ACGATGTGGTTCTCGCCTG | 59.72 | 60.08 | 276 | 132 |
100.00%
|
100.00%
|
| Primer 2 | Mycolicibacterium smegmatis | TTCCTGCCATGGGAGAAGC | ACGCGAAATTGCACGTGG | 59.70 | 59.75 | 157 | 123 |
100.00%
|
100.00%
|
| Primer 3 | Mycolicibacterium smegmatis | ACCATTCAGCAGCTGGTCC | AAGCGGTCACCTCAACCTG | 60.00 | 59.93 | 190 | 115 |
100.00%
|
100.00%
|
| Primer 4 | Mycolicibacterium smegmatis | AAGGCACTGATCGGACTGG | CGCGAGGTTGTTGACGTTC | 59.40 | 59.80 | 228 | 113 |
100.00%
|
100.00%
|
| Primer 5 | Mycolicibacterium smegmatis | TTCACCGTTGCGAACCTG | ACGATGTGGTTCTCGCCTG | 58.27 | 60.08 | 275 | 112 |
100.00%
|
100.00%
|
| Primer 6 | Mycolicibacterium smegmatis | TCCTGGGCATCCTCGACAT | TGGCGAACGACAGCTTGT | 60.38 | 59.89 | 273 | 108 |
100.00%
|
100.00%
|
| Primer 7 | Mycolicibacterium smegmatis | AAAACCCACACCGTCGACC | TGGCGAACGACAGCTTGT | 60.52 | 59.89 | 217 | 108 |
100.00%
|
100.00%
|
| Primer 8 | Mycolicibacterium smegmatis | TGGACAAGCTGGACACAGG | TCTCACCGATCCGCAGTTC | 59.55 | 59.49 | 207 | 106 |
100.00%
|
100.00%
|
| Primer 9 | Mycolicibacterium smegmatis | TGAAGGCACTGATCGGACTG | CGCGAGGTTGTTGACGTTC | 59.75 | 59.80 | 230 | 106 |
100.00%
|
100.00%
|
| Primer 10 | Mycolicibacterium smegmatis | GGCGTTCAACGTCATCACC | ACCATCACCGACGAGAAGC | 59.50 | 59.78 | 249 | 103 |
100.00%
|
100.00%
|